Gram Stain
Gram Stain : Chemistry and Chemical Disasters. A staining procedure used in classifying bacteria. A bacterial smear on a slide is stained with a purple basic triphenyl methane dye, usually crystal violet, in the presence of iodine/potassium iodide. The cells are then rinsed with alcohol or other solvent, and then counterstained, usually with safranin. The bacteria then appear purple or red according to their ability to keep the purple stain when rinsed with alcohol. This property is related to the composition of the bacterial cell wall
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